Journal:

Article Title: Synergistic effects of RXR? and PPAR? ligands to inhibit growth in human colon cancer cells--phosphorylated RXR? is a critical target for colon cancer management

doi: 10.1136/gut.2007.129858

Figure Lengend Snippet: Figure 1 Levels of the phosphorylated form of the retinoid X receptor α (RXRα) protein in colon cancer cell lines and the FHC normal human colon epithelial cell line. Seven human colon cancer cell lines Caco2, HT29, Colo201, Colo320, DLD‐1, HCT‐116 and SW837 cells and FHC normal human colon epithelial cells were treated with vehicle (lane 1), 5 μM 9‐cis‐retinoic acid (RA) (lane 2), 50 μM PD98059 (lane 3) or the combination of 50 μM PD98059 and 5 μM 9‐cisRA (lane 4) for 24 h. Total protein extracts were prepared from the indicated cell lines, and equivalent amounts of protein (15 μg/lane) were then examined by a western blot analysis for the phosphorylated RXRα protein. An antibody against glyceraldehyde‐3‐phosphate dehydrogenase served as a loading control. The intensities of blots were quantitated by densitometry, and the values are shown as bar graphs in the lower panel. Repeat western blot analyses gave similar results. The asterisks represent a significant difference(p<0.01) in comparison with column 1. Bars show the SD.

Article Snippet: The HCT‐116 human colorectal cancer cell line and the FHC normal human fetal colon cell line were obtained from ATCC (Manassas, VA, USA).

Techniques: Western Blot, Control, Comparison